5-Dehydroshikimate reductase in the tea plant (Camellia sinensis L.). Properties and distribution

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5-Dehydroshikimate reductase in the tea plant (Camellia sinensis L.). Properties and distribution.

1. A method for the extraction of 5-dehydroshikimate reductase (EC 1.1.1.25) from tea plant tissues in an active soluble state has been developed. It is dependent on the use, in the extraction medium, of an insoluble polyphenol adsorbent (Polyclar AT), which prevents the polyphenols present from precipitating all the proteins. 2. The enzyme has the following properties: pH optima at pH10.1 in g...

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Effects of light and darkness on polyphenol distribution in the tea plant (Camellia sinensis L.).

1. Flavonoid synthesis was able to proceed in darkness in young shoots and seedlings of the tea plant, but was increased by light. 2. The initial effect of darkness was to inhibit synthesis of the A ring or its linkage to the phenylpropane moiety of the flavonoid, but later the hydroxylation state of the flavanols was affected, leading to smaller proportions of gallocatechins and of complex leu...

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Differential Proteomic Analysis of Chilling Stress Responses in Tea Plant [Camellia sinensis (L.) O. Kuntze]

Low temperature is one of the serious environmental stresses affecting plant growth and agricultural production. It not only inhibits metabolic reactions but also causes osmotic, oxidative, and other damages [1]. Chilling temperatures that range from 0 to 12°C are common during the growing season and can substantially decrease plant productivity [2]. Plants respond and acclimate to chilling str...

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Fractionation and speciation of arsenic in three tea gardens soil profiles and distribution of As in different parts of tea plant (Camellia sinensis L.).

The distribution pattern and fractionation of arsenic (As) in three soil profiles from tea (Camellia sinensis L.) gardens located in Karbi-Anglong (KA), Cachar (CA) and Karimganj (KG) districts in the state of Assam, India, were investigated depth-wise (0-10, 10-30, 30-60 and 60-100 cm). DTPA-extractable As was primarily restricted to surface horizons. Arsenic speciation study showed the presen...

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Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways.

Leaves of tea (Camellia sinensis L.) contain extraordinary large amounts of (-)-epigallocatechin, (-)-epicatechin, (+)-gallocatechin, and (+)-catechin and derivatives of these compounds that show positive effects on human health. The health-promoting effects of flavan 3-ols, especially those of green tea, are of scientific and public interest. Furthermore, they play a crucial role in defense ag...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1966

ISSN: 0006-2936

DOI: 10.1042/bj0980248